|
KMID : 0812020130190020171
|
|
Korean Journal of Neurogastroenterology and Motility
2013 Volume.19 No. 2 p.171 ~ p.178
|
|
|
Validation of Octanoate Breath Test for Measuring Gastric Emptying in Rats
|
|
Demedts Ingrid
Vanormelingen Christophe
Billoen Hubert Van
Vanuytsel Tim
Farre Ricard
Masaoka Tatsuhiro
Verbruggen Alfons
Verbeke Kristien
Berghe Pieter Vanden
Tack Jan
|
|
|
Abstract
|
|
|
|
Background/Aims Lack of simple and repeatable tests hampers gastric emptying studies in rats. The aim of this study was to adapt the 14C-octanoate solid gastric emptying breath test for application in rats, and to validate it against radioscintigraphic method. Methods After ingestion of a meal containing 3 mCi 99mTc and 2 mCi 14C-octanoate, 23 male Wistar rats were placed on a gamma camera in a airflow container. Scintigraphic images were taken at regular intervals. The amount of 14CO2 in a regularly replaced hyamine hydroxide solution, capturing CO2 in the outflow air, was counted using liquid scintillation spectrometry. 99mTc gastric retention curves and 14CO2-excretion curves were fitted to their respective data. Three rats underwent the same procedures after administration of atropine. Results Overall Tr10% (time at which 10% of the original amount of 99mTc remained in the stomach) was 355 ± 64 minutes; Te90% (time at which 90% of total amount of 14CO2 was excreted) was 325 ± 106 minutes. Their correlation coefficient was 0.71, R-square 0.50 and P ＜ 0.005. Tr1/2 (50% of original amount of 99mTc remained) was 124 ± 28 minutes; Te1/2 (50% of total amount of 14CO2 excreted) 114 ± 32 minutes. Their correlation coefficient was 0.83 with R-square of 0.69 and P ＜ 0.00005. In 12 immobilized animals correlation was even better: correlation coefficient 0.84; R-square 0.71 and P ＜ 0.001 (Tr10% was 388 ± 117 minutes; Te90% 532 ± 219 minutes; Tr1/2 of 165 ± 54 minutes; Te1/2 of 175 ± 67 minutes). Atropine significantly lengthened all emptying times: 904 ± 307 and 1461 ± 684 minutes for Tr10% and Te90%, respectively; and 432 ± 117 minutes for Tr1/2 and 473 ± 190 minutes for Te1/2. Conclusions We adapted and validated the 14C-octanoate gastric emptying breath test for application in rats.
|
|
|
KEYWORD
|
|
|
Breath tests, 14C-octanoate, Gastric emptying, Rats
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|